Magnetically Bioprinted 3D Spheroid Co-cultures: A Method for Co-culturing Cancerous Cells and Fibroblasts
Published: April 30, 2023
Abstract
Source: Noel P. et al. Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts. J. Vis. Exp. (2017)
This video explains the 3D magnetic bioprinting of co-cultured pancreatic cancer cells and fibroblasts. Pancreatic cancer cells and activated pancreatic fibroblast cells are co-cultured and magnetized using a biocompatible nanoparticle assembly, to generate spheroids, under the influence of magnetic forces.
Protocol
1.Culture of Pancreatic Cancer 3D Spheroids using Magnetic Bioprinting Using standard aseptic tissue culture technique, culture cells of interest in a T75 flask to a confluency of 70-80% in appropriate growth media. NOTE: Typically, 5-7 × 106 cells from a 70-80% confluent T75 flask were harvested. Two different cell types were used in this study – Patu8902 (pancreatic tumor cells) and PS1 cells (activated pancreatic stellate cells). Both cell lines were cultured in…
開示
The authors have nothing to disclose.
Materials
| 0.05% Trypsin EDTA | Sigma | 25300-054 | |
| 96 well cell repellant plate | USA Scientific/ Griener Bio-One | 655970 | spheroid growth plate |
| Cellometry Cell counting slides | Nexcelom | CHT4-SD100-002 | |
| n3D Magnetic Spheroid drive | Nano3D Bioscience | 655841 | Part of Spheroid Bioprinting Kit |
| n3D Magnetic Spheroid holding drive |
Nano3D Bioscience | 655841 | Part of Spheroid Bioprinting Kit |
| n3D NanoShuttle-PL | Nano3D Bioscience | 655841 | Part of Spheroid Bioprinting Kit: store at 4 °C |
| Patu8902 cells | Laboratory Stock | pancreatic ductal adenocarcinoma cells |
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| PS1 cells | Laboratory Stock | activated pancreatic stellate cells | |
| RPMI1640 | Gibco | 11875-093 | growth media for cells, spheroids |
