Escape Response Assay: A Method to Study the Response of Zebrafish Larva to Touch Stimuli
Published: April 30, 2023
Abstract
Source: Sztal, T. E., et al. Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish. J. Vis. Exp. (2016)
This video describes the touch-evoke response assay, which measures the response of the zebrafish larva to touch stimuli.
Protocol
1. Touch-evoked Response Assay Preparation of 2 dpf Embryos for Touch-evoked Response Assay Ensure that the time of day at which the test is conducted is consistent between experiments because activity can vary dramatically throughout the day. NOTE: The experiment should be performed blinded and the order of testing randomized to minimize experimental artifacts. Assign the fish strains a number, which is unknown to the individual carrying out the experime…
Materials
| 90 mm Petri Dishes | Pacific Laboratory Products | PT S90001 | |
| Tweezers, style 8 | ProSciTech | T04-821 | |
| Incubator | Thermoline Scientific | TEI-43L | |
| High Speed Camera | Baumer | HXC20 | |
| StreamPix5 | NorPix | Step 1.2.3 | |
| Temperature Control Unit | Viewpoint | ||
| http://www.randomization.com | N/A | Steps 1.1.2 |
