Mouse Echocardiography: A Non-invasive Technique to Assess Cardiac Morphology
Published: April 30, 2023
Abstract
Source: Baudouy, D., et al. Echocardiographic and Histological Examination of Cardiac Morphology in the Mouse. J. Vis. Exp. (2017).
This video describes a protocol to perform echocardiography on a laboratory mouse.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Echocardiography Determine the body weight of the mouse using a standard laboratory balance while holding it lightly by the tail to ensure proper positioning. Anesthetize the animal by the intraperitoneal (i.p.) injection of 50 mg/kg pentobarbital. NOTE: Any other kind of anesthesia can…
Representative Results
Figure 1: Standard-resolution echocardiography (>10 MHz) to measure LV systolic and diastolic dimensions and cardiac function in mice with dilated cardiac hypertrophy after myocardial infarction versus control animals. Representative echocardiographic recordings from normal, healthy mice (A) and animals with LV dilation and reduced LV function after myoca…
Materials
| Ultrasound transmission gel; Gel Aquasonic 100 | Parker | ||
| Linear ultrasound probe; L15-7io | Philips Healthcare | ||
| Echocardiograph; IE33 xMATRIX | Philips Healthcare | ||
| Rodent shaver | Harvard Apparatus | 34-0243 |
