Assessing Gait by Footprint Analysis: A Method to Test Motor Coordination in Mice

Published: April 30, 2023

Abstract

Source: Sugimoto, H. and Kawakami, K. Low-cost Protocol of Footprint Analysis and Hanging Box Test for Mice Applied the Chronic Restraint Stress. J. Vis. Exp. (2019).

In this video, we describe the footprint testing procedure and related analysis to assess gait and motor coordination in mice.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Footprint Analysis Set up the Runway (Figure 1A) Cut a piece of white paper (29.7 cm x 42 cm x 0.09 mm) longitudinally into three lengths of equal width. Set a piece of white paper (9.9 cm x 42 cm) on the table. Put the dark goal box at the distal e…

Representative Results

Figure 1. Footprint analysis. (A) Runway for footprint analysis. A foot-painted mouse (forelimbs: red ink; hindlimbs: black ink) was allowed to walk from the proximal end to the goal box. (B) Representative image of footprint and measurement of parameters. Three measurements of each parameter (forelimb and hindlimb stride lengths, widths…

開示

The authors have nothing to disclose.

Materials

Goal box O’hara & Co. http://ohara-time.co.jp/products/balanced-beam-test/ Accessory for apparatus of balanced beam test
Boxes O’hara & Co. Side wall of runway
Black ink  Shin-asahi
Red ink Maruyamakogyo BC-6
Disposable Petri Dish Corning 351008 Petri dish (35 mm in diameter)
Askul Multipaper Super White J Monochrome A3 Askul 701-712 White paper (29.7 cm x 42 cm x 0.09mm) 

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記事を引用
Assessing Gait by Footprint Analysis: A Method to Test Motor Coordination in Mice. J. Vis. Exp. (Pending Publication), e20029, doi: (2023).

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