Barnes Maze: A Method For Assessing Spacial Learning and Memory in Lab Animals
Published: April 30, 2023
Abstract
Source: Ingersoll, J. et al. Analyzing Spatial Learning and Prosocial Behavior in Mice Using the Barnes Maze and Damsel-in-Distress Paradigms. J. Vis. Exp. (2018).
This video shows how to use the Barnes Maze with a mouse. The Barnes Maze testing procedure is used by researchers to assess spatial learning and memory in laboratory animals following repeated trials.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Basic Housing of Mice House the mice in plastic cages with solid bottoms and sides, and a layer of soft bedding and nesting material such as paper shredding. Use bedding that is composed of shredded corn cobs or wood shavings, and ensure that the bedding is changed regularly for sanitation. Provide access to f…
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The authors have nothing to disclose.
Materials
| Petri Dishes | Corning | 353025 | Spray painted and used as covers for Barnes maze holes |
| Plywood (3/4 in.) | LP Building Products | 22487 | To construct Barnes maze |
| Spray Paint | Krylon | 1274937 | Used to paint petri dish caps black, white paint used to paint plywood |
| Cup Hooks (5/8 in.) | Ace Hardware | 5360615 | 2 used on either side of ventral hole surfaces; rubberband wraps around hooks to hold cap flat |
| Poster Board | Creatology | Used at edges of maze as extra cues | |
| Light Bulbs | Phillips | 100W light bulb, used during the trials | |
| Rubber bands | Sprano Brand | Size 62, used to keep caps held to plywood | |
| Ultrasonic noisemaker | Victor mini PestChaser | M753SN | Used as aversive stimuli |
