Barnes Maze: A Method For Assessing Spacial Learning and Memory in Lab Animals

Published: April 30, 2023

Abstract

Source: Ingersoll, J. et al. Analyzing Spatial Learning and Prosocial Behavior in Mice Using the Barnes Maze and Damsel-in-Distress Paradigms. J. Vis. Exp. (2018).

This video shows how to use the Barnes Maze with a mouse. The Barnes Maze testing procedure is used by researchers to assess spatial learning and memory in laboratory animals following repeated trials.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Basic Housing of Mice House the mice in plastic cages with solid bottoms and sides, and a layer of soft bedding and nesting material such as paper shredding. Use bedding that is composed of shredded corn cobs or wood shavings, and ensure that the bedding is changed regularly for sanitation. Provide access to f…

開示

The authors have nothing to disclose.

Materials

Petri Dishes Corning 353025 Spray painted and used as covers for Barnes maze holes
Plywood (3/4 in.) LP Building Products 22487 To construct Barnes maze
Spray Paint Krylon 1274937 Used to paint petri dish caps black, white paint used to paint plywood
Cup Hooks (5/8 in.) Ace Hardware 5360615 2 used on either side of ventral hole surfaces; rubberband wraps around hooks to hold cap flat
Poster Board Creatology Used at edges of maze as extra cues
Light Bulbs Phillips 100W light bulb, used during the trials
Rubber bands Sprano Brand Size 62, used to keep caps held to plywood
Ultrasonic noisemaker Victor mini PestChaser M753SN Used as aversive stimuli

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記事を引用
Barnes Maze: A Method For Assessing Spacial Learning and Memory in Lab Animals. J. Vis. Exp. (Pending Publication), e20028, doi: (2023).

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