JOVE JOURNAL

発生生物学

Showing 481 - 492 in 797 videos.

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity

Shen Zhang¹, Yonggen Wu¹, Yimin Weng², Zhihui Xu¹, Wenmin Chen³, Dahan Zheng⁴, Wei Lin⁵, Jun Liu⁶, Ying Zhou^1,7

¹Reproductive Medicine Center,The First Affiliated Hospital of Wenzhou Medical University, ²Department of Orthopaedics,The Second Affiliated Hospital of Wenzhou Medical University, ³Department of Obstetrics,The First Affiliated Hospital of Wenzhou Medical University, ⁴School of Laboratory Medicine and Life Science,Wenzhou Medical University, ⁵School of Pharmaceutical Science,Wenzhou Medical University, ⁶Stem Cells and Genetic Engineering Group, AgriBioscience Research Centre,Department of Economic Development, Jobs, Transport and Resources, ⁷Department of Histology and Embryology,Wenzhou Medical University

Journal (Developmental Biology)

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

Deborah A. Goldspink¹, Zoe J. Matthews², Elizabeth K. Lund¹, Tom Wileman², Mette M. Mogensen¹

¹School of Biological Sciences,University of East Anglia, ²Norwich Medical School,University of East Anglia

Journal (Developmental Biology)

Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification

Dwayne B. Holmes¹, Vivi M. Heine^1,2

¹Department of Pediatrics/Child Neurology, Amsterdam Neuroscience,VU University Medical Center, ²Department of Complex Trait Genetics, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience,Vrije Universiteit Amsterdam

Journal (Developmental Biology)

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Felix Ellett¹, Daniel Irimia¹

¹BioMEMS Resource Center, Department of Surgery,Massachusetts General Hospital–Harvard Medical School–Shriners Burns Hospital

Journal (Developmental Biology)

Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

Adrienne T. Perkins¹, Sharon E. Bickel¹

¹Department of Biological Sciences,Dartmouth College

Journal (Developmental Biology)

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Matthias Becker^1,2, Janita A. Maring^1,2, Barbara Oberwallner², Benjamin Kappler², Oliver Klein^1,2, Volkmar Falk^2,3,4,5, Christof Stamm^2,3,4

¹Charité - Universitätsmedizin Berlin,corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, ²Berlin-Brandenburg Center for Regenerative Therapies (BCRT), ³German Center for Cardiovascular Research (DZHK), ⁴Deutsches Herzzentrum Berlin (DHZB), ⁵Department of Cardiovascular Surgery, Charité - Universitätsmedizin Berlin,corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

Journal (Developmental Biology)

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos

A.C. Berrun¹, D.L. Stachura¹

¹Department of Biological Sciences,California State University, Chico

Journal (Developmental Biology)

AFM and Microrheology in the Zebrafish Embryo Yolk Cell

Maria Marsal¹, Ignasi Jorba², Elena Rebollo¹, Tomas Luque², Daniel Navajas², Enrique Martín-Blanco¹

¹Instituto de Biología Molecular de Barcelona,Consejo Superior de Investigaciones Científicas, ²Institute for Bioengineering of Catalonia,Universitat de Barcelona and CIBER Enfermedades Respiratorias

Journal (Developmental Biology)

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Jaroslav Slamecka¹, Javier Laurini², Troy Shirley¹, Simon Philipp Hoerstrup³, Benedikt Weber^3,4,5, Laurie Owen¹, Steven McClellan¹

¹Mitchell Cancer Institute,University of South Alabama, ²College of Medicine,University of South Alabama, ³Institute for Regenerative Medicine,University of Zurich, ⁴皮膚学部,University Hospital Zurich, ⁵Center for Applied Biotechnology and Molecular Medicine (CABMM),University of Zurich - Irchel Campus

Journal (Developmental Biology)

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Cui Lin¹, Dongxing Zhu², Greg Markby¹, Brendan M. Corcoran³, Colin Farquharson¹, Vicky E. Macrae¹

¹Developmental Biology, The Roslin Institute and R(D)SVS,University of Edinburgh, ²Guangzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital, School of Basic Medical Sciences,Guangzhou Medical University, ³Clinical Sciences and R(D)SVS,University of Edinburgh

Journal (Developmental Biology)

Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice

Shuai Li¹, Wipawee Winuthayanon¹

¹School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine,Washington State University

Journal (Developmental Biology)

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Amanda N. Marra¹, Marisa Ulrich¹, Audra White¹, Meghan Springer¹, Rebecca A. Wingert¹

¹Department of Biological Sciences,University of Notre Dame

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