JOVE JOURNAL

生化学

Showing 265 - 276 in 776 videos.

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Katherina Hemmen*¹, Susobhan Choudhury*¹, Mike Friedrich¹, Johannes Balkenhol¹, Felix Knote¹, Martin J. Lohse², Katrin G. Heinze¹

¹Rudolf Virchow Center for Integrative and Translation Bioimaging,Julius-Maximilian University Wuerzburg, ²Max Delbrück Center for Molecular Medicine, Berlin

Journal (Biochemistry)

Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System

James B. Pace¹, Nian N. Huang¹, Jacqueline P. Séguin¹, Candi Esquina¹, Erin Olin¹, Guangyu Zhu¹, Grant Carr¹

¹Discovery Biology,Curia Global Inc.

Journal (Biochemistry)

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Sergey I. Kovalchuk¹, Rustam Ziganshin¹, Irina Shelukhina²

¹Laboratory of Bioinformatics Methods in Combinatorial Chemistry and Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences, ²Department of Molecular Neuroimmune Signalling, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences

Journal (Biochemistry)

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

Chloe D. Truong^1,2, Dewight R. Williams³, Mary Zhu¹, Joseph Che-Yen Wang⁴, Po-Lin Chiu^1,2

¹School of Molecular Sciences,Arizona State University, ²Center for Applied Structural Discovery, Biodesign Institute,Arizona State University, ³Eyring Materials Center,Arizona State University, ⁴Department of Microbiology and Immunology,The Pennsylvania State University

Journal (Biochemistry)

Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography

Jana Moravcová¹, Matyáš Pinkas¹, Radka Holbová¹, Jiří Nováček¹

¹Central European Institute of Technology,Masaryk University

Journal (Biochemistry)

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Yue Zhang*¹, Peng Cao*², Fang Lu¹, Jinjun Cheng³, Huihua Qu⁴

¹School of Life Science,Beijing University of Chinese Medicine, ²Third Affiliated Hospital, Beijing University of Chinese Medicine, ³National Institute of TCM Constitution and Preventive Medicine,Beijing University of Chinese Medicine, ⁴Center of Scientific Experiment,Beijing University of Chinese Medicine

Journal (Biochemistry)

Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy

David P. Klebl¹, Frank Sobott², Howard D. White³, Stephen P. Muench¹

¹School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology,University of Leeds, ²School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology,University of Leeds, ³Department of Physiological Sciences,Eastern Virginia Medical School

Journal (Biochemistry)

In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase

Victoria Lucia Alonso^1,2, Romina Manarin¹, Virginia Perdomo¹, Ernesto Gulin³, Esteban Serra^1,2, Pamela Cribb^1,2

¹Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas,Universidad Nacional de Rosario (UNR), ²Instituto de Biología Molecular y Celular de Rosario (IBR),CONICET, ³Instituto de Investigaciones Biomédicas (INBIOMED),Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

Journal (Biochemistry)

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Claudia Neves Correa^1,2, Louise Oliveira Fiametti¹, Maria Eduarda Mazzi Esquinca¹, Leandro Mantovani de Castro^1,2

¹Department of Biological and Environmental Sciences, Bioscience Institute,Sao Paulo State University (UNESP), ²Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute,Sao Paulo State University (UNESP)

Journal (Biochemistry)

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

Jessica M. Lewis*¹, Pauline M. L. Coulon*¹, Taylor A. McDaniels*¹, Nichollas E. Scott¹

¹Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,The University of Melbourne

Journal (Biochemistry)

Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot

Yu Han^1,3, Cody T. Thomas^1,3, Sara A. Wennersten^1,3, Edward Lau^1,3, Maggie P. Y. Lam^1,2,3

¹Department of Medicine-Cardiology,University of Colorado Anschutz Medical Campus, ²Department of Biochemistry & Molecular Genetics,University of Colorado Anschutz Medical Campus, ³Consortium for Fibrosis Research & Translation, School of Medicine,University of Colorado Anschutz Medical Campus

Journal (Biochemistry)

Quaternary Structure Modeling Through Chemical Cross-Linking Mass Spectrometry: Extending TX-MS Jupyter Reports

Hamed Khakzad^1,2, Swen Vermeul³, Lars Malmström^4,5,6

¹Equipe Signalisation Calcique et Infections Microbiennes,Ecole Normale Supérieure Paris-Saclay, ²Institut National de la Santé et de la Recherche Médicale, ³Scientific IT Services,ETH Zurich, ⁴Institute for Computational Science,University of Zurich, ⁵S3IT,University of Zurich, ⁶Division of Infection Medicine, Department of Clinical Sciences Lund, Faculty of Medicine,Lund University

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