1John T. Milliken Department of Medicine, Division of Nutritional Sciences and Obesity Medicine,Washington University 医学部, 2Nutrition and Obesity Research Center, Cellular and Molecular Biology Core,Washington University 医学部, 3Nutrition and Obesity Research Center, Animal Model Research Core,Washington University 医学部
概要
Here, we describe a comprehensive method for measuring mitochondrial oxidative phosphorylation in fresh permeabilized skeletal muscle fibers from either human or mouse muscle. This method allows for the real-time quantification of mitochondrial respiration and the assessment of fuel preference and metabolic flexibility while preserving existing mitochondrial networks and membrane integrity.
