1Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 2Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 3Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 4Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
概要
Presentiamo un protocollo per l’assemblaggio modulare basato su CRISPR (CRISPRmass), un metodo per la costruzione ad alto rendimento di librerie plasmidiche UAS-cDNA/ORF in Drosophila utilizzando risorse cDNA/ORF disponibili pubblicamente. CRISPRmass può essere applicato alla modifica di varie librerie di plasmidi.
