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Studying ER Stress and UPR Activation During HIV-1 Infection in T-Cells

Studying ER Stress and UPR Activation During HIV-1 Infection in T-Cells

June 14, 2024

Author Spotlight: Exploring the Role of Unfolded Protein Response in HIV-1 Replication and Infectivity

01:33 min
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329 Views

Quantitation of HIV-1 Infection in TZM-bl Cells and Infection in CEM-GFP T-Cell Line

01:58 min
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83 Views

Thioflavin T Staining for Assessing Endoplasmic Reticulum Stress in CEM-GFP Cells

01:48 min
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58 Views

Determining Activation and Expression of Unfolded Protein Response Markers in HIV-1 Infected CEM-GFP Cells

01:52 min
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23 Views

shRNA-Mediated Gene Knockdown in HEK-293T and Jurkat J6 Cells, Treatment with ER Stress Inducer, and Analyzing its Effect on HIV-1 Replication

03:08 min
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50 Views
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1National Centre for Cell Science,SP Pune University Campus

概要

Here, we describe some established methods to determine endoplasmic reticulum (ER) stress and unfolded protein response (UPR) activation, with particular emphasis on HIV-1 infection. This article also describes a set of protocols to investigate the effect of ER stress/UPR on HIV-1 replication and virion infectivity.

Article