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Microfluidic Droplet Assay for Single-Cell Cytokine Secretion Analysis

Microfluidic Droplet Assay for Single-Cell Cytokine Secretion Analysis

March 08, 2024

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

02:07 min
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Observation Chamber Fabrication for Imaging Single-Cell Suspensions and Magnetic Nanoparticles

01:50 min
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Preparation of Functionalized Magnetic Nanoparticles for Time-Resolved Fluorescent Imaging

01:47 min
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Encapsulation of the Cells and Nanoparticles into Droplets for Fluorescent Imaging

04:11 min
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1Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences,ETH Zürich, 2Department of Biomedicine,Aarhus University, 3Laboratory for Tumor and Stem Cell Dynamics, Institute of Molecular Health Sciences, Department of Biology,ETH Zürich

概要

The protocol describes an advanced microfluidic platform to quantitatively measure cytokine secretion dynamics of individual human peripheral blood mononuclear cells. The platform measures up to three cytokines in parallel (IL-6, TNFα, and IL-1β) for each individual cell stimulated with lipopolysaccharide as an example.

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