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生化学
MyDoBID- A Novel Technique for Identifying Muscle Fiber Types Efficiently

MyDoBID- A Novel Technique for Identifying Muscle Fiber Types Efficiently

September 22, 2023

Author Spotlight: Deciphering the Mysteries of Skeletal Muscle Fiber Types Using the MyDoBID Technique

02:11 min
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1,437 Views

Isolation of Single-Fiber from Freeze-Dried Human Skeletal Muscle

01:15 min
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269 Views

MHCI and MHCII Analysis of Single Fibers Isolated from Freeze-Dried Muscle Samples Using Dot Blot

04:08 min
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224 Views
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1Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, School of Agriculture, Biomedicine and Environment,La Trobe University

概要

This protocol demonstrates single-fiber isolation from freeze-dried human skeletal muscle and fiber-type classification according to Myosin heavy chain (MHC) isoform using the dot blotting technique. Identified MHC I and II fiber samples can then be further analyzed for fiber type-specific differences in protein expression using western blotting.

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