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Development of pHluorin2-Based Sensor for Glycosomal pH in Trypanosoma brucei
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Author Spotlight: Advancements in Glycosomal pH Monitoring in Trypanosoma brucei Using pHluorin2 Biosensor
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Measuring Dynamic Glycosomal pH Changes in Living Trypanosoma brucei

Author Spotlight: Advancements in Glycosomal pH Monitoring in Trypanosoma brucei Using pHluorin2 Biosensor

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01:46 min

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January 19, 2024

DOI:

10.3791/66279-v

DOI:
10.3791/66279-v

•

01:46 min
January 19, 2024

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10 Views
, , , , , ,
1Department Chemistry and Biochemistry,Brigham Young University, 2Eukaryotic Pathogens Innovation Center,Clemson University, 3Department of Genetics and Biochemistry,Clemson University, 4Department of Physics and Astronomy,Clemson University

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Trypanosoma BruceiGlycosomePH BiosensorPHluorin2Glucose MetabolismFlow CytometryPH RegulationGlycolysisHexokinasePhosphofructokinase

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Call, D., Pizarro, S. S., Tovey, E., Knight, E., Baumgardner, C., Christensen, K. A., Morris, J. C. Measuring Dynamic Glycosomal pH Changes in Living Trypanosoma brucei. J. Vis. Exp. (203), e66279, doi:10.3791/66279 (2024).

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