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Un modello di asfissia perinatale da maialino per studiare la lesione cardiaca e l'emodinamica dopo l'arresto cardiaco, la rianimazione e il ritorno della circolazione spontanea
JoVE Journal
Medicina
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JoVE Journal Medicina
A Piglet Perinatal Asphyxia Model to Study Cardiac Injury and Hemodynamics after Cardiac Arrest, Resuscitation, and the Return of Spontaneous Circulation
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Un modello di asfissia perinatale da maialino per studiare la lesione cardiaca e l'emodinamica dopo l'arresto cardiaco, la rianimazione e il ritorno della circolazione spontanea

1,349 Views

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10:55 min

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January 13, 2023

DOI:

10.3791/64788-v

DOI:
10.3791/64788-v

•

10:55 min
January 13, 2023

•

5 Views
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1Institute of Clinical Medicine, Faculty of Medicine,University of Oslo, 2Department of Neonatal Intensive Care, Division of Pediatric and Adolescent Medicine,Oslo University Hospital Rikshospitalet, 3Department of Pediatric Research, Division of Paediatric and Adolescent Medicine,Oslo University Hospital Rikshospitalet, 4Department of Pediatrics,Vestfold Hospital Trust, 5Department of Anesthesiology,Oslo University Hospital Rikshospitalet, 6Department of Pediatrics, Faculty of Medicine and Dentistry,University of Alberta, 7Centre for the Studies of Asphyxia and Resuscitation, Neonatal Research Unit,Royal Alexandra Hospital, 8Division of Paediatric and Adolescent Medicine, Institute of Clinical Medicine,University of Oslo, 9Department of Paediatrics and Adolescent Health,University of Botswana, 10Department of Pediatric Research, Oslo University Hospital,University of Oslo, 11Department of Pediatrics, Robert H Lurie Medical Research Center,Northwestern University Chicago

Summary

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Questo modello di suinetto prevede strumentazione chirurgica, asfissia fino all'arresto cardiaco, rianimazione e osservazione post-rianimazione. Il modello consente il campionamento multiplo per animale e, utilizzando la pressione arteriosa invasiva continua, l'ECG e il monitoraggio non invasivo della gittata cardiaca, fornisce conoscenze sull'emodinamica e sulla fisiopatologia cardiaca nell'asfissia perinatale e nella rianimazione cardiopolmonare neonatale.

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Keyword Extraction:Piglet Perinatal Asphyxia ModelCardiac InjuryHemodynamicsCardiac ArrestResuscitationReturn Of Spontaneous CirculationNeonatal Cardiopulmonary ResuscitationPulseless Electrical ActivitySurgical InterventionInternal Jugular VeinCentral Venous CatheterFentanyl IVBalanced Carbohydrate Electrolyte Solution

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Stenersen, E. O., Olsen, A., Melheim, M., Solberg, R., Dannevig, I., Schmölzer, G., Cheung, P., Nakstad, B., Saugstad, O. D., Rønnestad, A., Solevåg, A. L. A Piglet Perinatal Asphyxia Model to Study Cardiac Injury and Hemodynamics after Cardiac Arrest, Resuscitation, and the Return of Spontaneous Circulation. J. Vis. Exp. (191), e64788, doi:10.3791/64788 (2023).

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