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使用改良的夹心 ELISA 定量来自中性粒细胞胞外陷阱的髓过氧化物酶 DNA 和中性粒细胞弹性蛋白酶 DNA 复合物
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA
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使用改良的夹心 ELISA 定量来自中性粒细胞胞外陷阱的髓过氧化物酶 DNA 和中性粒细胞弹性蛋白酶 DNA 复合物

DOI:
10.3791/64644-v

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07:18 min

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May 12, 2023

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, , , ,
1Department of Biochemistry and Biotechnology,University of Science and Technology Chittagong (USTC), 2Department of Emergency and Critical Care Medicine,Aichi Medical University

Capitoli

  • 00:04Introduction
  • 01:18Sample Collection and Storage
  • 02:13Modified Sandwich Enzyme‐Linked Immunosorbent Assay
  • 05:42Results: Myeloperoxidase‐DNA (MPO‐DNA) and Neutrophil Elastase‐DNA (NE‐DNA) Complex Quantification
  • 06:54Conclusion

Summary

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我们提出了一种改进的夹心酶联免疫吸附测定技术方案，以定量测量中性粒细胞细胞外捕获残留物的两种成分，髓过氧化物酶偶联-DNA 和中性粒细胞弹性蛋白酶偶联 DNA 复合物，来源于活化的中性粒细胞。

Tags

Myeloperoxidase-DNANeutrophil Elastase-DNANETsELISASepsisARDSDamage-associated Molecular PatternsPolymorphonuclear NeutrophilsPMADNase IEDTA

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