Rivista
/
Ricerca sul cancro
/
인간 조혈 줄기 및 전구 세포에서 발암성 이형접합체 기능 획득 돌연변이 공학
JoVE Journal
Ricerca sul cancro
This content is Free Access.
JoVE Journal Ricerca sul cancro
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
Please note that all translations are automatically generated. Click here for the English version.

인간 조혈 줄기 및 전구 세포에서 발암성 이형접합체 기능 획득 돌연변이 공학

2,801 Views

•

12:04 min

•

March 10, 2023

DOI:

10.3791/64558-v

DOI:
10.3791/64558-v

•

12:04 min
March 10, 2023

•

5 Views
, , , ,
1Division of Hematology, Department of Internal Medicine,Medical University of Graz, 2Division of Hematology, Stanford Cancer Institute, Stanford Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 3Department of Blood Group Serology and Transfusion Medicine,Medical University of Graz

Tags

CRISPR-Cas9Gain-of-function MutationsHematopoietic Stem And Progenitor CellsLeukemic TransformationFluorescent ReporterHeterozygous MutationsHomology-directed RepairGene EditingMolecular CloningCodon Optimization

Read Article

Cite this Article

Sconocchia, T., Foßelteder, J., Köhnke, T., Majeti, R., Reinisch, A. Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells. J. Vis. Exp. (193), e64558, doi:10.3791/64558 (2023).

Download .ris file

Copy

Share Video

.

Copy