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ヒト造血幹細胞および前駆細胞における発癌性ヘテロ接合性機能獲得変異の改変
JoVE Journal
Ricerca sul cancro
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JoVE Journal Ricerca sul cancro
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
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ヒト造血幹細胞および前駆細胞における発癌性ヘテロ接合性機能獲得変異の改変

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12:04 min

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March 10, 2023

DOI:

10.3791/64558-v

DOI:
10.3791/64558-v

•

12:04 min
March 10, 2023

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5 Views
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1Division of Hematology, Department of Internal Medicine,Medical University of Graz, 2Division of Hematology, Stanford Cancer Institute, Stanford Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 3Department of Blood Group Serology and Transfusion Medicine,Medical University of Graz

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CRISPR-Cas9Gain-of-function MutationsHematopoietic Stem And Progenitor CellsLeukemic TransformationFluorescent ReporterHeterozygous MutationsHomology-directed RepairGene EditingMolecular CloningCodon Optimization

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Cite this Article

Sconocchia, T., Foßelteder, J., Köhnke, T., Majeti, R., Reinisch, A. Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells. J. Vis. Exp. (193), e64558, doi:10.3791/64558 (2023).

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