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工程人造血干细胞和祖细胞中的致癌杂合功能获得突变
JoVE Journal
Ricerca sul cancro
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JoVE Journal Ricerca sul cancro
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
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工程人造血干细胞和祖细胞中的致癌杂合功能获得突变

2,801 Views

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12:04 min

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March 10, 2023

DOI:

10.3791/64558-v

DOI:
10.3791/64558-v

•

12:04 min
March 10, 2023

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5 Views
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1Division of Hematology, Department of Internal Medicine,Medical University of Graz, 2Division of Hematology, Stanford Cancer Institute, Stanford Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 3Department of Blood Group Serology and Transfusion Medicine,Medical University of Graz

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CRISPR-Cas9Gain-of-function MutationsHematopoietic Stem And Progenitor CellsLeukemic TransformationFluorescent ReporterHeterozygous MutationsHomology-directed RepairGene EditingMolecular CloningCodon Optimization

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Cite this Article

Sconocchia, T., Foßelteder, J., Köhnke, T., Majeti, R., Reinisch, A. Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells. J. Vis. Exp. (193), e64558, doi:10.3791/64558 (2023).

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