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工程人造血干细胞和祖细胞中的致癌杂合功能获得突变
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March 10, 2023
DOI:
1Division of Hematology, Department of Internal Medicine,Medical University of Graz, 2Division of Hematology, Stanford Cancer Institute, Stanford Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 3Department of Blood Group Serology and Transfusion Medicine,Medical University of Graz
Summary
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忠实地模拟造血干细胞和祖细胞（HSPC）体细胞突变的新策略对于更好地研究造血干细胞生物学和血液恶性肿瘤是必要的。在这里，描述了通过结合使用CRISPR / Cas9和双rAAV供体转导来模拟HSPC中杂合功能获得突变的方案。
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Sconocchia, T., Foßelteder, J., Köhnke, T., Majeti, R., Reinisch, A. Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells. J. Vis. Exp. (193), e64558, doi:10.3791/64558 (2023).
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