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基于深度学习的冷冻电子断层扫描
JoVE Journal
Biologia
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JoVE Journal Biologia
Deep Learning-Based Segmentation of Cryo-Electron Tomograms
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基于深度学习的冷冻电子断层扫描

6,858 Views

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10:25 min

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November 11, 2022

DOI:

10.3791/64435-v

DOI:
10.3791/64435-v

•

10:25 min
November 11, 2022

•

102 Views
, , , , ,
1Pennsylvania State University-College of Medicine, 2Object Research Systems

Summary

Automatically generated

这是一种使用一个断层图的一部分作为训练输入来训练多层 U-Net 以对冷冻电子断层图进行多类分割的方法。我们描述了如何将该网络推断为其他断层图，以及如何提取分割以进行进一步分析，例如断层图平均和细丝追踪。

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Cite this Article

Heebner, J. E., Purnell, C., Hylton, R. K., Marsh, M., Grillo, M. A., Swulius, M. T. Deep Learning-Based Segmentation of Cryo-Electron Tomograms. J. Vis. Exp. (189), e64435, doi:10.3791/64435 (2022).

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