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Reconstitution of Actin-Based Motility with Commercially Available Proteins
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Reconstitution of Actin-Based Motility with Commercially Available Proteins
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October 28, 2022
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This protocol describes how to produce actin comets on the surfaces of beads using commercially available protein ingredients. Such systems mimic the protrusive structures found in cells, and can be used to examine physiological mechanisms of force production in a simplified way.
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Sykes, C., Plastino, J. Reconstitution of Actin-Based Motility with Commercially Available Proteins. J. Vis. Exp. (188), e64261, doi:10.3791/64261 (2022).
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