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Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon
1Laboratory of Genetics,The Salk Institute for Biological Studies, 2Jack H. Skirball Center for Chemical Biology and Proteomics,The Salk Institute for Biological Studies, 3Department of Chemistry and Biochemistry,University of California San Diego, 4Graduate School of Biological Sciences, Section of Molecular Biology,University of California San Diego, 5Department of Integrative Structural and Computational Biology,The Scripps Research Institute
Capitoli
- 00:05Introduction
- 00:28Setting Up the Tilted Data Collection
- 03:23Results: Images of the Grid at Square Magnification with Different Tilt Angles and Hole Exposures and 2D Class Averages Collected at Different Tilt Angles
- 04:27Conclusion
The present protocol describes a generalized and easy-to-implement scheme for tilted single-particle data collection in cryo-EM experiments. Such a procedure is especially useful for obtaining a high-quality EM map for samples suffering from preferential orientation bias due to adherence to the air-water interface.
