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Ganzhirn-Einzelzellbildgebung und Analyse intakter neonataler Mausgehirne mittels MRT, Gewebereinigung und Lichtblattmikroskopie
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1UNC Neuroscience Center,University of North Carolina, Chapel Hill, 2Department of Genetics,University of North Carolina, Chapel Hill, 3Department of Psychiatry,University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging,The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center,The University of North Carolina at Chapel Hill, 6Department of Neurology,The University of North Carolina at Chapel Hill, 7Department of Computer Science,The University of North Carolina at Greensboro
Capitoli
- 00:05Introduction
- 00:48Light-Sheet Imaging
- 02:25Image Processing Using NuMorph
- 04:12Image Analysis
- 06:26Results: Quantification of Nuclear Markers Within Mouse Brain After Imaging on a Light-Sheet Microscope Using NuMorph
- 07:57Conclusion
Dieses Protokoll beschreibt Methoden zur Durchführung von Magnetresonanztomographie, Clearing und Immunmarkierung intakter Mausgehirne mit iDISCO+, gefolgt von einer detaillierten Beschreibung der Bildgebung mittels Lichtblattmikroskopie und nachgeschalteten Analysen mit NuMorph.
Tags
Keywords:
Whole-brainSingle-cellImagingAnalysisIntactNeonatalMouseBrainMRILight-sheet MicroscopyImage AnalysisNuclei QuantificationComputational PipelinePreprocessingCell DetectionImaging TimeComputational ResourcesSample MountingSample OrientationZoom MagnificationLight SheetNumerical ApertureDynamic FocusingLaser PowerLight Sheet WidthTile OverlapCondaNuMorphMATLABImage ProcessingIntensity Adjustment
