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Whole-Brain Single-Cell Imaging en Analyse van Intacte Neonatale Muizenhersenen Met behulp van MRI, Tissue Clearing en Light-Sheet Microscopie
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1UNC Neuroscience Center,University of North Carolina, Chapel Hill, 2Department of Genetics,University of North Carolina, Chapel Hill, 3Department of Psychiatry,University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging,The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center,The University of North Carolina at Chapel Hill, 6Department of Neurology,The University of North Carolina at Chapel Hill, 7Department of Computer Science,The University of North Carolina at Greensboro
Capitoli
- 00:05Introduction
- 00:48Light-Sheet Imaging
- 02:25Image Processing Using NuMorph
- 04:12Image Analysis
- 06:26Results: Quantification of Nuclear Markers Within Mouse Brain After Imaging on a Light-Sheet Microscope Using NuMorph
- 07:57Conclusion
Dit protocol beschrijft methoden voor het uitvoeren van magnetische resonantie beeldvorming, clearing en immunolabeling van intacte muizenhersenen met behulp van iDISCO +, gevolgd door een gedetailleerde beschrijving van beeldvorming met behulp van light-sheet microscopie en downstream analyses met behulp van NuMorph.
Tags
Whole-brainSingle-cellImagingAnalysisIntactNeonatalMouseBrainMRILight-sheet MicroscopyImage AnalysisNuclei QuantificationComputational PipelinePreprocessingCell DetectionImaging TimeComputational ResourcesSample MountingSample OrientationZoom MagnificationLight SheetNumerical ApertureDynamic FocusingLaser PowerLight Sheet WidthTile OverlapCondaNuMorphMATLABImage ProcessingIntensity Adjustment
