/

Immunologia e infezioni

/

针对 1 型糖尿病、乳糜泻和 COVID-19 的高通量多重筛查

JoVE Journal
Immunologia e infezioni

This content is Free Access.

JoVE Journal Immunologia e infezioni

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Please note that all translations are automatically generated. Click here for the English version.

针对 1 型糖尿病、乳糜泻和 COVID-19 的高通量多重筛查

DOI:

10.3791/63787-v

•

06:46 min

•

July 05, 2022

•

Xiaofan Jia*¹, Ling He*^1,2, Dongmei Miao, Caiguo Zhang, Marian Rewers, Liping Yu

¹Barbara Davis Center for Diabetes,University of Colorado School of Medicine, ²Department of Endocrinology, Guangzhou First People’s Hospital, School of Medicine,South China University of Technology

Capitoli

00:04Introduction
01:06Create Linker‐Coupled Antigen Solution
02:36Preparation of Antigen Labeled Serum and the 6‐Plex Plate
03:54Serum/Antigen Incubates Transfer into the 6‐Plex Plate
04:42Washing the 6‐Plex Plate and Reading Buffer Addition
05:11Results: Comparison of Antibody Levels Study Between the Single and the 6‐Plex ECL Assay
05:59Conclusion

Summary

Traduzione automatica

根据2019年筛查1型糖尿病、乳糜泻和冠状病毒病的迫切需要，我们开发了一种高通量6-Plex电化学发光测定法，可同时检测所有四种胰岛自身抗体、组织谷氨酰胺转移酶自身抗体和针对严重急性呼吸综合征冠状病毒2受体结合域的抗体。

Tags

Multiplex Assay Autoantibody Screening Type 1 Diabetes Celiac Disease COVID-19 GAD65 SARS-CoV-2 RBD IA-2 TTG ZnT8 Proinsulin Biotinylated Antigen Ruthenium-labeled Antigen High-throughput Screening Early Disease Prediction Precise Diagnosis

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

加速1型糖尿病感应在小鼠体内的过继转移致糖尿病的CD4 + T细胞

用于适应高通量/高含量筛查的细胞内分枝杆菌定量的微观表型检测

高通量筛选RNA病毒的广谱化学抑制剂

高通量设置的建立筛选小分子能调节C-二GMP信令<em>绿脓杆菌</em

基于荧光的淋巴细胞检测适用于小分子的高通量筛选

无细胞生化荧光酶法测定高密度脂蛋白脂质过氧化的高通量测定

高通量的高效液氧活化病毒膜融合检测 HIV-1 病毒膜融合抑制剂的研究

沙门氏菌筛选的高通量平台.

一种高通量志贺氏菌特异性杀菌试验

非酒精性脂肪性肝炎合并2型糖尿病的一种先进的小鼠模型

Read Article