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Organoidi intestinali canini in un sistema di supporto permeabile a doppia camera
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Organoidi intestinali canini in un sistema di supporto permeabile a doppia camera
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March 02, 2022
DOI:
1Department of Biomedical Sciences, College of Veterinary Medicine,Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University, 33D Health Solutions Inc., 4Office of New Animal Drug Evaluation,Center for Veterinary Medicine, Food and Drug Administration, 5Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences,Center for Drug Evaluation and Research, Food and Drug Administration
Summary
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Qui, presentiamo un protocollo che descrive la coltura di organoidi intestinali canini in un sistema di supporto permeabile a doppia camera. Vengono descritti la semina organoide nei supporti permeabili, il mantenimento del monostrato e i successivi esperimenti di permeabilità ai farmaci.
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Cite this Article
Gabriel, V., Zdyrski, C., Sahoo, D. K., Dao, K., Bourgois-Mochel, A., Atherly, T., Martinez, M. N., Volpe, D. A., Kopper, J., Allenspach, K., Mochel, J. P. Canine Intestinal Organoids in a Dual-Chamber Permeable Support System. J. Vis. Exp. (181), e63612, doi:10.3791/63612 (2022).
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