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이중 챔버 투과성 지원 시스템의 송곳니 장 오가노이드
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11:11 min
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March 02, 2022
DOI:
1Department of Biomedical Sciences, College of Veterinary Medicine,Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University, 33D Health Solutions Inc., 4Office of New Animal Drug Evaluation,Center for Veterinary Medicine, Food and Drug Administration, 5Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences,Center for Drug Evaluation and Research, Food and Drug Administration
Summary
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여기에서, 우리는 이중 챔버, 투과성 지원 시스템에서 송곳니 장 오가노이드의 배양을 설명하는 프로토콜을 제시합니다. 투과성 지지체에서의 오가노이드 시딩, 단층 유지, 및 후속 약물 투과성 실험이 기재되어 있다.
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Cite this Article
Gabriel, V., Zdyrski, C., Sahoo, D. K., Dao, K., Bourgois-Mochel, A., Atherly, T., Martinez, M. N., Volpe, D. A., Kopper, J., Allenspach, K., Mochel, J. P. Canine Intestinal Organoids in a Dual-Chamber Permeable Support System. J. Vis. Exp. (181), e63612, doi:10.3791/63612 (2022).
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