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大鼠星形胶质细胞和小胶质细胞的原代培养及其在肌萎缩侧索硬化症研究中的应用
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Neuroscienze
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JoVE Journal Neuroscienze
Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis
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大鼠星形胶质细胞和小胶质细胞的原代培养及其在肌萎缩侧索硬化症研究中的应用

DOI:
10.3791/63483-v

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09:36 min

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June 23, 2022

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, , ,
1Center for Laser Microscopy, Institute for Physiology and Biochemistry “Jean Giaja”,University of Belgrade Faculty of Biology

Capitoli

  • 00:04Introduction
  • 01:04Primary Cell Culture Preparation
  • 04:21Microglial Cell Culture
  • 05:26Time-Lapse Video Imaging
  • 06:36Data Analysis
  • 07:34Results: Characterization of Different Glial Cell Types in Culture
  • 08:43Conclusion

Summary

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我们在这里提出了一个关于如何从大鼠皮质制备神经胶质细胞、星形胶质细胞和小胶质细胞的原代培养物的协议，用于细胞内 Ca2+ 的延时视频成像，用于研究 hSOD1G93A 大鼠模型中肌萎缩侧索硬化症的病理生理学。

Tags

Keywords: Primary Astrocyte CulturePrimary Microglia CultureCalcium SignalingAmyotrophic Lateral SclerosisNeuroinflammatory DiseasesPersonalized MedicineCell IsolationCell CultureDMEMFBSAntibiotics

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