Rivista
/
Biologia dello sviluppo
/
Isolatie van rat vetweefsel Mesenchymale stamcellen voor differentiatie in insulineproducerende cellen
JoVE Journal
Biologia dello sviluppo
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
Please note that all translations are automatically generated. Click here for the English version.

Isolatie van rat vetweefsel Mesenchymale stamcellen voor differentiatie in insulineproducerende cellen

6,270 Views

•

08:09 min

•

August 29, 2022

DOI:

10.3791/63348-v

DOI:
10.3791/63348-v

•

08:09 min
August 29, 2022

•

14 Views
, , ,
1Biochemistry Department, Faculty of Pharmacy,Ain Shams University, 2Pharmacology and Biochemistry Department, Faculty of Pharmacy,The British University in Egypt, 3Center for Drug Research and Development (CDRD), Faculty of Pharmacy,The British University in Egypt

Summary

Automatically generated

Vetweefsel-afgeleide mesenchymale stamcellen (Ad-MSC's) kunnen een potentiële bron zijn van MSC's die differentiëren in insulineproducerende cellen (IPC's). In dit protocol bieden we gedetailleerde stappen voor de isolatie en karakterisering van epididymale Ad-MSC's bij ratten, gevolgd door een eenvoudig, kort protocol voor het genereren van IPC's van dezelfde rat Ad-MSC's.

Explore More Videos

Mesenchymal Stem CellsIsolationCharacterizationDifferentiationInsulin-producing CellsCollagenase DigestionCD MarkersMesodermal Lineages

Read Article

Cite this Article

Kassem, D. H., Habib, S. A., Badr, O. I., Kamal, M. M. Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells. J. Vis. Exp. (186), e63348, doi:10.3791/63348 (2022).

Download .ris file

Copy

Share Video

.

Copy