/
/
Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
2,053 Views
•
09:28 min
•
August 25, 2022
DOI:
1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine,University of Toronto, 2Krembil Research Institute, Toronto Western Hospital,University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital,Sinai Health Systems, 4Medical Oncology and Hematology,Princess Margaret Cancer Centre
Summary
Automatically generated
This is a step-by-step guide for using a commercially available rotary cell culture system to culture lymphocytes in simulated microgravity using specialized disposable culture vessels. This culturing method may be applied to any suspension-type cell culture.
Explore More Videos
LymphocytesSimulated MicrogravityRotary Cell Culture SystemImmune CellsSpace EnvironmentHuman PhysiologyMolecular LevelEconomically FeasibleCell CultureControlTreatmentComplete MediaIncubatorFlask ControlT25 Suspension Culture FlaskStock Cell CultureSyringe Port CapsStopcocksFill PortSerological PipetteVacuum System
Read Article
Cite this Article
de Korte, M., Keating, A., Wang, C. Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System. J. Vis. Exp. (186), e63296, doi:10.3791/63296 (2022).
Copy