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Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

DOI:
10.3791/63296-v

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09:28 min

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August 25, 2022

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, ,
1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine,University of Toronto, 2Krembil Research Institute, Toronto Western Hospital,University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital,Sinai Health Systems, 4Medical Oncology and Hematology,Princess Margaret Cancer Centre

Capitoli

  • 00:04Introduction
  • 00:48Preparation of Vessels for Cell Culture
  • 01:43Loading Vessels with Stock Cell Culture
  • 04:42Removing Bubbles from the Vessels
  • 05:47Attaching the Vessel to the Rotating Base
  • 06:36Treatment and Harvesting Cells from the Vessels
  • 08:06Results: Application of the Rotary Cell Culture System to Study the Effects of Simulated Microgravity on Lymphocytes
  • 08:55Conclusion

Summary

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This is a step-by-step guide for using a commercially available rotary cell culture system to culture lymphocytes in simulated microgravity using specialized disposable culture vessels. This culturing method may be applied to any suspension-type cell culture.

Tags

LymphocytesSimulated MicrogravityRotary Cell Culture SystemImmune CellsSpace EnvironmentHuman PhysiologyMolecular LevelEconomically FeasibleCell CultureControlTreatmentComplete MediaIncubatorFlask ControlT25 Suspension Culture FlaskStock Cell CultureSyringe Port CapsStopcocksFill PortSerological PipetteVacuum System

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