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Neuroscience

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JoVE Journal Neuroscience
Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
 

Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator

Article DOI: 10.3791/63073-v • 07:47 min • October 20th, 2021
October 20th, 2021 •
1, 1, 2, 1
1Department of Medical Cell Biology, Institute for Anatomy and Cell Biology, Heidelberg University, 2Department of Neurogenetics, Max-Planck-Institute for Experimental Medicine

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This article describes a protocol to determine differences in basal redox state and redox responses to acute perturbations in primary hippocampal and cortical neurons using confocal live microscopy. The protocol can be applied to other cell types and microscopes with minimal modifications.

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Live Imaging Mitochondrial Glutathione Redox State Primary Neurons Ratiometric Indicator Pathophysiological Conditions Treatment Strategies Protecting Mitochondria Organ-specific Assessment Dynamic Tracing Changes Real Time Additional Indicators Mitochondrial Membrane Potential Calcium Concentrations Culture Cells Tissue Explants Slice Cultures Scanning Confocal Microscope Settings Detector Settings Objective Selection Coverslip Mounting Imaging Buffer Microscope Setup
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