Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / A Three-Dimensional Spheroid Model to Investigate …
JoVE Journal
Cancer Research

Questo Contenuto è ad Accesso gratuito.

JoVE Journal Cancer Research
A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma
 

A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma

Article DOI: 10.3791/62868-v • 12:24 min • September 30th, 2021
September 30th, 2021 •
*1,2, *2, 2, 2, *3, *4,5
1Department of Biochemistry, Faculty of Pharmacy, Minia University, 2National Institute for Health Research Birmingham Liver Biomedical Research Unit and Centre for Liver and Gastrointestinal Research, Institute of Immunology and Immunotherapy, University of Birmingham, 3Newcastle Fibrosis Research Group, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, 4Newcastle University Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, 5The Liver Unit, Department of Medicine, Freeman Hospital, Newcastle-upon-Tyne Hospitals NHS Foundation Trust
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

Comprehensive in vitro models that faithfully recapitulate the relevant human disease are lacking. The current study presents three-dimensional (3D) tumor spheroid creation and culture, a reliable in vitro tool to study the tumor-stromal interaction in human hepatocellular carcinoma.

Tags

3D Tumor Spheroids Tumor-stromal Interaction Hepatocellular Carcinoma Hanging Droplets Method Cell Culture Tumor Microenvironment Spatial Interactions Drug Screening Tool Pharmacological Effects Spheroid Formation Image Analysis Software Huh7 Cell Line Hep3B Cell Line COS-7 Cell Line LX2 Fibroblast Cell Line
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter