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用于宿主靶向治疗结核病临床前检测的自动化培养系统
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09:34 min
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August 16, 2021
DOI:
1Department of Clinical Medicine, Trinity Translational Medicine Institute, St. James’s Hospital, Trinity College Dublin,The University of Dublin, 2School of Pharmacy and Biomolecular Sciences,Royal College of Surgeons in Ireland (RCSI), 3SFI Advanced Materials and Bioengineering Research (AMBER) Centre,RCSI & TCD, 4SFI Centre for Research in Medical Devices (CURAM), RCSI,Dublin and National University of Ireland
Summary
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快速有效地定量细胞内 结核分枝杆菌 的生长对于寻求改进的结核病（TB）疗法至关重要。该协议描述了一种基于肉汤的比色检测测定，使用自动液体培养系统来量化用候选宿主定向疗法处理的巨噬细胞中的Mtb生长。
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Cite this Article
O’Leary, S., Bahlool, A. Z., O’Connor, G., Cryan, S., Keane, J. M., O’Sullivan, M. P. An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis. J. Vis. Exp. (174), e62838, doi:10.3791/62838 (2021).
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