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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
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Biochimica
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JoVE Journal
Biochimica
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
DOI:
10.3791/62754-v
•
10:10 min
•
November 13, 2021
•
Yue Zhang*
1
,
Peng Cao*
2
,
Fang Lu
,
Jinjun Cheng
,
Huihua Qu
1
School of Life Science
,
Beijing University of Chinese Medicine
,
2
Third Affiliated Hospital, Beijing University of Chinese Medicine
,
3
National Institute of TCM Constitution and Preventive Medicine
,
Beijing University of Chinese Medicine
,
4
Center of Scientific Experiment
,
Beijing University of Chinese Medicine
Capitoli
00:01
Introduction
01:16
Preparation and Characterisation of Colloidal Gold
02:40
Synthesis of the AuNP-mAB Conjugate
05:01
Assembly of the strip
08:15
Representative Results
09:28
Conclusion
Tags
Lateral Flow Immunochromatographic Strip
Small Molecule Compounds
Colloidal Gold
Chloroauric Acid
Sodium Citrate
Conjugate Pad
Nitrocellulose Membrane
Absorbent Membrane
Sample Pad
Gold Nanoparticles
UV Spectroscopy
TEM
PH Optimization
Antibody Conjugation
Article
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