Rivista
/
Biologia
/
三维类器官和球状体模型的染色和高分辨率成像
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models
Please note that all translations are automatically generated. Click here for the English version.

三维类器官和球状体模型的染色和高分辨率成像

10,228 Views

•

07:35 min

•

March 27, 2021

DOI:

10.3791/62280-v

DOI:
10.3791/62280-v

•

07:35 min
March 27, 2021

•

41 Views
, , , , , , , ,
1Childhood Cancer & Cell Death (C3),Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), Lyon 69373, 2Plateforme Anatomopathologie Recherche,Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), 3Plateforme d’Imagerie cellulaire, Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), 4PerkinElmer S.A.S.

Summary

Automatically generated

在这里，我们提供了详细、稳健和互补的方案，对从 100 μm 到几毫米的固定三维细胞培养模型进行染色和亚细胞分辨率成像，从而能够可视化它们的形态、细胞类型组成和相互作用。

Explore More Videos

3D In Vitro ModelsOrganoidsSpheroidsWhole Mount StainingSubcellular Resolution ImagingPermeabilizationPrimary AntibodySecondary AntibodyHoechst 333423D Structure ManipulationPipettingCentrifugationGentle Agitation

Read Article

Cite this Article

Gonzalez, A. L., Luciana, L., Le Nevé, C., Valantin, J., Francols, L., Gadot, N., Vanbelle, C., Davignon, L., Broutier, L. Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models. J. Vis. Exp. (169), e62280, doi:10.3791/62280 (2021).

Download .ris file

Copy

Share Video

.

Copy