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Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
JoVE Journal
Biologia
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JoVE Journal Biologia
Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

DOI:
10.3791/62257-v

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09:19 min

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February 15, 2021

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, , , , ,
1Leibniz Institute on Aging, Fritz-Lipmann-Institute

Capitoli

  • 00:04Introduction
  • 00:50Preparing Pipettes for Trituration of the Collagenase-Digested Muscles
  • 01:54EDL Muscle Isolation and Collagenase Digestion
  • 03:19Myofiber Dissociation and Culture
  • 04:20siRNA Transfection, Fixation, and IF Staining
  • 06:31Results: Culture and Transfection of Murine EDL Muscle-Derived Single Myofibres
  • 08:34Conclusion

Summary

Traduzione automatica

Traduzione automatica

In this protocol an in vitro culturing and functional analysis method for muscle stem cells is described, which preserves most of their interactions with their endogenous niche.

Tags

Muscle Stem CellsSingle Myofiber CultureEx VivoNicheSiRNA TransfectionPasteur PipettesHorse SerumEthanolTibialis AnteriorEDL MuscleDigestionIsolation MediumMyofibers

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