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iPSC-巨噬细胞死亡神经母细胞的体外定量成像检测

JoVE Journal
Neuroscienze

This content is Free Access.

JoVE Journal Neuroscienze

In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages

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iPSC-巨噬细胞死亡神经母细胞的体外定量成像检测

4,941 Views

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09:48 min

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February 14, 2021

DOI:

10.3791/62217-v

DOI:

10.3791/62217-v

•

09:48 min

February 14, 2021

•

10 Views

Hazel Hall-Roberts^1,2,3, Elena Di Daniel², William S. James¹, John B. Davis², Sally A. Cowley¹

¹James Martin Stem Cell Facility, Sir William Dunn School of Pathology,University of Oxford, ²Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building,University of Oxford, ³UK Dementia Research Institute,Cardiff University

Summary

神经退行性疾病与受管制的微胶质功能障碍有关。本文概述了iPSC巨噬细胞对神经母细胞的体外测定。定量显微镜读数用于活细胞延时成像和固定细胞高含量成像。

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