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一种基于DNA/Ki67的流式细胞术测定法，用于接种疫苗的小鼠抗原特异性CD8 T细胞的细胞周期分析
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January 05, 2021
DOI:
1Institute of Molecular Biology and Pathology,National Research Council of Italy (CNR), 2Department of Molecular Medicine,University of Rome “Sapienza”, 3Center for Life Nano Science,Istituto Italiano di Tecnologia, 4Department of Molecular Medicine and Medical Biotechnology,University of Naples Federico II, 5Reithera Srl, 6IRCCS, Neuromed
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克隆扩增是抗原特异性T细胞反应的关键特征。然而，抗原反应T细胞的细胞周期研究不佳，部分原因是技术限制。我们描述了一种流式细胞术方法，用于分析接种疫苗小鼠的脾脏和淋巴结中的克隆扩增抗原特异性CD8 T细胞。
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Simonetti, S., Natalini, A., Peruzzi, G., Nicosia, A., Folgori, A., Capone, S., Santoni, A., Di Rosa, F. A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice. J. Vis. Exp. (167), e61867, doi:10.3791/61867 (2021).
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