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Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs
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November 19, 2020
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Co-translational insertion into pre-formed nanodiscs makes it possible to study cell-free synthesized membrane proteins in defined lipid environments without contact with detergents. This protocol describes the preparation of essential system components and the critical parameters for improving expression efficiency and sample quality.
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Levin, R., Koeck, Z., Dötsch, V., Bernhard, F. Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs. J. Vis. Exp. (165), e61844, doi:10.3791/61844 (2020).
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