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Transillumination-Assisted Dissectie van specifieke stadia van de muis seminifereuze epitheelcyclus voor downstream immunostaininganalyses
This content is Free Access.
1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit,University of Turku, Turku, Finland, 2Department of Pediatrics,Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health,Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute,Monash University, Melbourne, VIC 3800, Australia
Capitoli
- 00:04Introduction
- 00:51Testis Dissection and Preparation
- 02:10Transillumination-Assisted Microdissection
- 02:47Squash Preparation and Stage Verification
- 04:04Whole-Mount Seminiferous Tubule Immunostaining
- 07:04Results: Representative Mouse Seminiferous Epithelial Cycle Staging
- 09:16Conclusion
Dit protocol beschrijft transillumination-ondersteunde microdissectie van segmenten van volwassen muis seminifereuze tubuli die specifieke stadia van seminifereuze epitheelcyclus vertegenwoordigen, en celtypen daarin, en daaropvolgende immunostaining van squashpreparaten en intacte tubulussegmenten.
