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蠕虫对齐和Worm_CP，两个开源管道，用于从卡诺哈布迪蒂斯精英获得的荧光图像数据的直化 和定量
JoVE Journal
Biologia
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JoVE Journal Biologia
Worm-align and Worm_CP, Two Open-Source Pipelines for Straightening and Quantification of Fluorescence Image Data Obtained from Caenorhabditis elegans
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蠕虫对齐和Worm_CP，两个开源管道，用于从卡诺哈布迪蒂斯精英获得的荧光图像数据的直化 和定量

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11:16 min

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May 28, 2020

DOI:

10.3791/61136-v

DOI:
10.3791/61136-v

•

11:16 min
May 28, 2020

•

1 Views
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1Babraham Institute

Summary

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蠕虫对齐/Worm_CP 是一个简单的 FIJI/CellProfiler 工作流程，可用于拉直和对齐 卡诺哈布迪蒂斯埃利根样本 ，并评分全蠕虫图像为基础的检测，而无需事先的培训步骤。我们应用蠕虫对Worm_CP/分析方法，用于在活动物中对热休克诱导表达的定量，或在固定样品中对脂滴进行定量分析。

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Okkenhaug, H., Chauve, L., Masoudzadeh, F., Okkenhaug, L., Casanueva, O. Worm-align and Worm_CP, Two Open-Source Pipelines for Straightening and Quantification of Fluorescence Image Data Obtained from Caenorhabditis elegans. J. Vis. Exp. (159), e61136, doi:10.3791/61136 (2020).

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