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Intra-Arterial Delivery of Neural Stem Cells to the Rat and Mouse Brain: Application to Cerebral Ischemia
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JoVE Journal Neuroscienze
Intra-Arterial Delivery of Neural Stem Cells to the Rat and Mouse Brain: Application to Cerebral Ischemia

Intra-Arterial Delivery of Neural Stem Cells to the Rat and Mouse Brain: Application to Cerebral Ischemia

DOI:
10.3791/61119-v

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14:53 min

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June 26, 2020

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, , ,
1College of Public Health,Shaanxi University of Chinese Medicine, 2Spinal Cord and Brain Injury Research Center, Department of Physiology,University of Kentucky

Capitoli

  • 00:00Introduction
  • 00:24Preparation of Injection Cathedters and Surgical Hooks
  • 02:48Culture of GFP (+) Neural Stem Cells (NSCs)
  • 03:52Ischemic Stroke Surgery
  • 09:24NSC Injection
  • 13:07Representative Results
  • 14:24Conclusion

Summary

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A method for delivering neural stem cells, adaptable for injecting solutions or suspensions, through the common carotid artery (mouse) or external carotid artery (rat) after ischemic stroke is reported. Injected cells are distributed broadly throughout the brain parenchyma and can be detected up to 30 d after delivery.

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Intra-arterial DeliveryNeural Stem CellsCerebral IschemiaStrokeCatheter DesignSurgical HooksStem Cell InjectionGFP-positive Neural Stem Cells

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