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Metingen van fysiologische stressreacties in C. Elegans
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Biologia
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JoVE Journal Biologia
Measurements of Physiological Stress Responses in C. Elegans
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Metingen van fysiologische stressreacties in C. Elegans

DOI:
10.3791/61001-v

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10:36 min

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May 21, 2020

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, , , , , , ,
1Department of Molecular and Cell Biology,University of California, Berkeley

Capitoli

  • 00:04Introduction
  • 01:00Stress Response Induction: hsp-4p::GFP Readout
  • 02:11Stereo Microscope or Low-Magnification Wide-Field/Compound Microscope Imaging
  • 04:33Quantitative Reporter Measurement
  • 06:54Stress and Heat Sensitivity Measurement
  • 07:54Results: Representative Cellular Stress Response Evaluation
  • 09:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

Hier karakteriseren we cellulaire proteotoxische stressreacties in de nematode C. elegans door het meten van de activering van fluorescerende transcriptieverslaggevers en assaying gevoeligheid voor fysiologische stress.

Tags

Keywords: C. ElegansPhysiological Stress ResponsesStress Response AssayHeat Shock ProteinEndoplasmic Reticulum Unfolded Protein ResponseGFP ReporterSodium AzideMicromanipulationHigh-throughputGenetic PerturbationsPharmacological PerturbationsMolecular And Physiological AnalysisSynchronizationImagingMicroscopy

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