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Standardized Histomorphometric Evaluation of Osteoarthritis in a Surgical Mouse Model
1Center for Orthopedic Research and Translational Sciences, Department of Orthopedics and Rehabilitation,Pennsylvania State College of Medicine, 2Department of Biochemistry and Molecular Biology,Pennsylvania State College of Medicine, 3Department of Pharmacology,Pennsylvania State College of Medicine
Capitoli
- 00:04Introduction
- 01:13Histomorphometric Analysis Setup
- 01:59Total, Calcified, and Uncalcified Cartilage Area Measurements
- 02:47Chondrocyte Number and Phenotype Evaluation
- 03:17Tibial Articular Surface Perimeter Measurement
- 03:47Subchondral Bone and Marrow Space Area Measurement
- 04:30Anterior and Posterior Osteophyte Area and Synovial Thickness Measurement
- 05:21Results: Representative Histomorphometric Osteoarthritis (OA) Evaluation in Destabilization of the Medial Meniscus (DMM) and Sham Mice
- 07:06Conclusion
The current protocol establishes a rigorous and reproducible method for quantification of morphological joint changes that accompany osteoarthritis. Application of this protocol can be valuable in monitoring disease progression and evaluating therapeutic interventions in osteoarthritis.
