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Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
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Biochimica
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JoVE Journal Biochimica
Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

5,599 Views

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10:12 min

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March 25, 2020

DOI:

10.3791/60734-v

DOI:
10.3791/60734-v

•

10:12 min
March 25, 2020

•

9 Views
, , , , ,
1Department of Pharmacology, Addiction Science and Toxicology,The University of Tennessee HSC, 2Department of Chemistry,University of Illinois at Chicago

Summary

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Two methods of cholesterol enrichment are presented: the application of cyclodextrin saturated with cholesterol to enrich mammalian tissues and cells, and the use of cholesterol-enriched phospholipid-based dispersions (liposomes) to enrich Xenopus oocytes. These methods are instrumental for determining the impact of elevated cholesterol levels in molecular, cellular, and organ function.

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Cite this Article

Slayden, A., North, K., Bisen, S., Dopico, A. M., Bukiya, A. N., Rosenhouse-Dantsker, A. Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol. J. Vis. Exp. (157), e60734, doi:10.3791/60734 (2020).

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