Rivista
/
Genetica
/
Transcriptome-Wide Profilering van Protein-RNA Interacties door Cross-Linking en Immunoprecipitatie Gemedieerd door FLAG-Biotine Tandem Purification
JoVE Journal
Genetica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Genetica
Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification
Please note that all translations are automatically generated. Click here for the English version.

Transcriptome-Wide Profilering van Protein-RNA Interacties door Cross-Linking en Immunoprecipitatie Gemedieerd door FLAG-Biotine Tandem Purification

5,678 Views

•

08:46 min

•

May 18, 2020

DOI:

10.3791/60730-v

DOI:
10.3791/60730-v

•

08:46 min
May 18, 2020

•

3 Views
, ,
1Tsinghua-Peking Center for Life Sciences, School of Medicine and School of Life Sciences,Tsinghua University, 2Department of Molecular Biology,University of Texas Southwestern Medical Center

Summary

Automatically generated

Hier presenteren we een gewijzigd CLIP-seq protocol genaamd FbioCLIP-seq met FLAG-biotine tandem zuivering om de RNA doelen van RNA-bindende eiwitten (RBPs) in zoogdiercellen te bepalen.

Explore More Videos

Protein-RNA InteractionsCLIP-seqFLAG-BiotinTranscriptome-wide ProfilingRBPsMammalian CellsDirect Protein-bound RNAsIsotope-freeSDS-PAGEMembrane TransferMouse Embryonic Stem CellsUV Cross-linkingCell LysisDNase I TreatmentFLAG BeadsFLAG Peptide Elution

Read Article

Cite this Article

Bi, X., Zhang, X., Shen, X. Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification. J. Vis. Exp. (159), e60730, doi:10.3791/60730 (2020).

Download .ris file

Copy

Share Video

.

Copy