Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Katerina Korelova; Marketa Jirouskova; Lenka Sarnova; Martin Gregor

doi:10.3791/60507

/

Isolement et 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Please note that all translations are automatically generated. Click here for the English version.

Isolement et 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

10,790 Views

•

10:12 min

•

December 20, 2019

DOI:

10.3791/60507-v

DOI:

10.3791/60507-v

•

10:12 min

December 20, 2019

•

9 Views

Katerina Korelova¹, Marketa Jirouskova¹, Lenka Sarnova¹, Martin Gregor¹

¹Laboratory of Integrative Biology,Institute of Molecular Genetics of the Czech Academy of Sciences

Summary

Présenté ici est un protocole pour l'isolement des hépatocytes de souris des foies adultes de souris utilisant une technique modifiée de perfusion de collagène. On décrit également la culture à long terme des hépatocytes dans un arrangement 3D de sandwich de collagène aussi bien que l'immunolabeling des composants cytosquelettiques pour étudier la formation canaliculaire biliaire et sa réponse au traitement.

Explore More Videos

3D Collagen Sandwich Culture Primary Mouse Hepatocytes Bile Canalicular Formation Cytoskeleton Liver Isolation Hepatocyte Isolation In Vitro Studies Perfusion Heparin DMEM Collagen I PBS Cell Culture

Read Article

Isolement et 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Summary

Automatically generated

Explore More Videos