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在Vivo光学钙成像学习诱导突触可塑性在果蝇黑色素增生
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JoVE Journal Neuroscienze
In Vivo Optical Calcium Imaging of Learning-Induced Synaptic Plasticity in Drosophila melanogaster
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在Vivo光学钙成像学习诱导突触可塑性在果蝇黑色素增生

DOI:
10.3791/60288-v

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06:35 min

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October 08, 2019

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, ,
1Department of Molecular Neurobiology of Behavior,University of Göttingen

Capitoli

  • 00:04Titolo
  • 00:26In Vivo Calcium Imaging Preparation
  • 02:44In Vivo Calcium Imaging
  • 03:39Olfactory Conditioning and Odor-Evoked Calcium Transients Visualization
  • 05:07Results: Representative Postsynaptically-Localized Calcium and Learning-Induced Postsynaptic Plasticity Visualization
  • 05:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们提出了一个协议，其中前和/或午睡钙可以在果蝇学习和记忆的上下文中可视化。体内钙成像使用显着局部钙传感器与经典的嗅觉调节范式相结合，因此可以确定这种类型的关联学习背后的突触可塑性。

Tags

Keywords: In Vivo Optical Calcium ImagingSynaptic PlasticityDrosophila MelanogasterAssociative TrainingGenetically Encoded Calcium IndicatorImaging ChamberOdor DeliveryRinger’s SolutionDissectionCuticle RemovalNeuronal Responses

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