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Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease
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JoVE Journal Immunologia e infezioni
Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease
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Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease

DOI:
10.3791/60083-v

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08:05 min

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March 17, 2020

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, , , , , , , ,
1Cancer Division, Burnett School of Biomedical Sciences,University of Central Florida, 2Department of Biochemistry,Hanoi University of Pharmacy, 3Center for Cardiovascular Regeneration, Department of Cardiovascular Sciences,Houston Methodist Research Institute, 4Department of Epidemiology,University of Pittsburgh Graduate School of Public Health, 5Division of Cancer Control and Population Sciences,University of Pittsburgh Medical Center, Hillman Cancer Center

Capitoli

  • 00:04Introduction
  • 00:49Graft-Versus-Host Disease (GVHD) Induction
  • 02:53Donor T Cell Preparation
  • 04:54Bone Marrow-Derived Dendritic Cell (BM-DC) Preparation
  • 05:58Results: Representative Effects of Dendritic Cell Function in GVHD
  • 07:26Conclusion

Summary

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Traduzione automatica

Graft-versus-host disease is a major complication after allogeneic bone marrow transplantation. Dendritic cells play a critical role in the pathogenesis of graft-versus-host disease. The current article describes a novel bone marrow transplantation platform to investigate the role of dendritic cells in the development of graft-versus-host disease and the graft-versus-leukemia effect.

Tags

Bone Marrow TransplantationDendritic CellsGraft-versus-host DiseaseT-cell DepletionCell IsolationCell CultureImmune Cell PopulationsMacrophagesNeutrophilsCell RegenerationBone Marrow PreparationCell HarvestingCell StrainingCell WashingCell ResuspensionCell IncubationCell CountingCell Pooling

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